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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Cell cytoskeleton and proliferation study for the RANKL‐induced RAW264.7 differentiation
doi: 10.1111/jcmm.16390
Figure Lengend Snippet: Cell culture and induction agent information
Article Snippet:
Techniques: Cell Culture, Modification
Journal: BMJ Open Diabetes Research & Care
Article Title: Glycolytic overload-driven dysfunction of periodontal ligament fibroblasts in high glucose concentration, corrected by glyoxalase 1 inducer
doi: 10.1136/bmjdrc-2020-001458
Figure Lengend Snippet: Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament fibroblasts (PDLFs) for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture medium (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Article Snippet: Primary human PDLFs (cat. no. 2630) and Modified
Techniques: Concentration Assay, In Vitro, Derivative Assay